Examine the cross sections of the spinal cord with the 4x objective first. Locate all major regions as listed below. At higher power, note cell structure of central canal, posterior and anterior gray horns, and funiculi. Then illustrate first at lowest power and label the following:
Slide 4: Spinal Cord cross section and longitudinal sections, H-Eosin (H 1537)
Make four illustrations:
Click on images to enlarge:
overview: anterior median fissure, 100x:
anterior median fissure, 400x: posterior median sulcus, 80x: | Spinal Cord cross section (40x): (MF 4th : pages 99, 101) anterior median fissure
Here is a labeled view of the spinal cord.
|
| a: Central Canal (400x)
ependymal cells cilia grey commissure lumen Here is a labeled view of the central canal. |
| b: Anterior horn cell (400x)
clear hillock nucleus nucleolus Nissl bodies Here is a labeled view of anterior horn cells. |
| c: myelinated fibers (See MF 4 th:93-95) (400x)
(cross section at posterior funiculus) multiple cross sections of fibers myelin sheath axon |
| Slide 5: Spinal Cord with Dorsal Root Ganglion cross section,
Ag stain (H 1560) (MF 4 th: page 97) Single large illustration showing all of the following (40x): Here is a labeled view of the spinal cord with dorsal root ganglion. |
| spinal cord general features:
posterior gray horn anterior gray horn anterior median fissure posterior median sulcus dorsal root ganglion |
| dorsal root ganglion features:
ganglion cells nerve fibers epineurium (capsule) dorsal root if present: ventral root spinal nerve Here is a labeled view of the dorsal root ganglion.. |
Examine the cross sections of the spinal cord with the 4x objective first. Locate all major regions as listed below. At higher power, note cell structure of central canal, posterior and anterior gray horns, and funiculi. Then illustrate first at lowest power and label the following:
Slide 4: Spinal Cord cross section and longitudinal sections, H-Eosin (H 1537)
Make four illustrations:
Click on images to enlarge:
overview: anterior median fissure, 100x:
anterior median fissure, 400x: posterior median sulcus, 80x: | Spinal Cord cross section (40x): (MF 4th : pages 99, 101) anterior median fissure
Here is a labeled view of the spinal cord.
|
| a: Central Canal (400x)
ependymal cells cilia grey commissure lumen Here is a labeled view of the central canal. |
| b: Anterior horn cell (400x)
clear hillock nucleus nucleolus Nissl bodies Here is a labeled view of anterior horn cells. |
| c: myelinated fibers (See MF 4 th:93-95) (400x)
(cross section at posterior funiculus) multiple cross sections of fibers myelin sheath axon |
| Slide 5: Spinal Cord with Dorsal Root Ganglion cross section,
Ag stain (H 1560) (MF 4 th: page 97) Single large illustration showing all of the following (40x): Here is a labeled view of the spinal cord with dorsal root ganglion. |
| spinal cord general features:
posterior gray horn anterior gray horn anterior median fissure posterior median sulcus dorsal root ganglion |
| dorsal root ganglion features:
ganglion cells nerve fibers epineurium (capsule) dorsal root if present: ventral root spinal nerve Here is a labeled view of the dorsal root ganglion.. |
This detail shows the ventral root where it is formed from fibers from the anterior gray horn
Additional images of the spinal cord:
detail shows the ventral root where it is formed from fibers from the anterior gray
hornAdditional images of the spinal cord: